Nutrient agar | Principle | Composition |Preparation

Summary :

◉ Overview

◉ Composition of nutrient agar

◈ The composition can be adjusted to obtain optimal performance, for 1 liter of medium:

Note: Nutrient agar and nutrient broth share almost the same media composition. The main difference between them is that the nutrient agar contains a solidifying agent, the agar powder that causes the medium to solidify at room temperature.

◉ Preparation of nutrient agar

Note : Nutrient agar with 0.8% NaCl and pH 6.0 is used for the culture of bacteria requiring slightly acidic conditions. Nutrient media can be used as enriched media by the addition of 10% blood or other biological fluids such as ascetic fluid, serum, etc.

pseudomonas Nutrient agar

Pseudomonas in Nutrient agar

serratia Nutrient agar

Serratia in Nutrient agar

◈ Some colonies may have characteristic pigmentation


  1. Difco™ & BBL™ Manual, 2nd Edition
  2. BBL Nutrient agar- 2006
  3. thermofisher : nutrient agar
  4. oxoide : NUTRIENT AGAR
  5. merck millipore : Nutrient Agar pH 6.0 with 0.8% NaCl
  6. thermofisher : Nutrient Agar