Brain Heart Infusion Broth : Composition, Preparation and Uses

Summary :

◉ Overview

◉ Preparation / composition of BHIB

Suspend the components, dehydrated powder, in water (37 g in 1000 ml of purified/distilled water). Bring to a boil and completely dissolve the medium.
Distribute in test tubes and sterilize in an autoclave at 121°C for 15 minutes.

◉ Principle of Brain Heart Infusion Broth

This culture medium is highly nutritious and buffered to support the growth of fastidious and nonfastidious microorganisms, including aerobic and anaerobic bacteria.

Enzymatic digestion of animal tissues and brain-heart infusion provide amino acids, nitrogen, carbon, vitamins and minerals for the growth of organisms.

Glucose is the source of carbohydrates that microorganisms use by fermentative action.

Sodium chloride maintains the osmotic balance of the medium.

Disodium phosphate is the buffering agent.

◉ Interpretation

◈ No turbidity: no growth

◈ Turbidity: growth (presence of organisms)

bhi broth