Sabouraud Dextrose Agar (SDA)

☰ Summary :

Ⅰ. Overview

Note :The maltose in the original formula is less used today than glucose (dextrose) so the medium is commonly referred to as "Sabouraud's dextrose agar".

Ⅱ. Composition of Sabouraud Dextrose Agar

Suspend 65.0 grams in 1000 ml of purified/distilled water. Bring to a boil to completely dissolve the medium. Sterilize by autoclaving at 15 pounds (121°C) pressure for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri dishe.

Ⅲ. Different types of Sabouraud agar

🏾 Sabouraud agar with antibiotics

Commonly used antibiotics are gentamicin, which inhibits gram-negative bacteria, and/or chloramphenicol, which inhibits a wide range of gram-positive and gram-negative fungi, and cycloheximide, which primarily inhibits saprophytic fungi but not dermatophytes or yeasts.

  • Chloramphenicol can have an inhibitory effect on several pathogenic fungi.
  • Penicillin, gentamicin, and streptomycin, or a combination of these, have been shown to be effective in inhibiting bacteria without affecting fungal growth

Antibiotics should only be added after the medium has been autoclaved and then cooled to approximately 45-50°C.

🏾 Sabouraud agar with methyl blue

Sabouraud Methyl Blue Agar contains 0.01% methyl blue in addition to Sabouraud Agar. This medium has been reported to distinguish between C. albicans and C. dubliniensis based on the yellow fluorescence of C. albicans under a Woods lamp

🏾 Emmon’s Modification of Sabouraud’s Agar

In 1977, Emmons formulated an alternative version of Sabouraud's agar, he modified the formula by adjusting the pH and reducing the dextrose concentration. This neutral pH promotes the recovery of certain pathogenic fungi.

🏾 Sabouraud Broth

The same composition of SDA but without agar.

Ⅳ. Results

The yeasts develop creamy to white colonies. The molds will grow as filamentous colonies of different colors. Count the number of colonies and take into account the dilution factor (if the sample to be tested has been diluted) to determine the number of yeasts and/or molds per gram or milliliter of material.

Sabouraud Dextrose Agar

Candida auris on Sabouraud agar (source :@in_petri)

Sabouraud Dextrose Agar

Aspergillus niger on Sabouraud agar (source :@smallbutcool)


  1. F. C. ODDS - Sabouraud('s) agar
  2. neogen - Sabouraud Dextrose Agar
  3. Liofilchem - Sabouraud Dextrose Agar
  4. HUMEAU - Sabouraud Dextrose Agar
  5. HIMEDIA - Sabouraud Dextrose Agar
  6. thermofisher - Simmons Citrate Agar (Dehydrated)
  7. BD - Sabouraud Glucose Agarl
  8. ASM - Sabouraud Agar for Fungal Growth Protocols
  9. Handbook of MICROBIOLOGICAL MEDIA Fourth Edition
  10. Manual of Commercial Methods in Clinical Microbiology 2ed