Bacterial identification flowchart

Bacteria are identified in laboratories by various methods, including microscopy ( fresh state, after staining ), observation of growth characteristics (list of culture media), determination of reactions to organic and inorganic compounds (API Gallery, Microbiological techniques) and molecular techniques.

The following figures illustrate decision algorithms to facilitate bacterial identification .


Identification of Gram positive bacilli


Identification of Gram positive bacilli

Identification of Gram-positive Cocci


Identification of Gram-positive Cocci

Identification of positive ONPG Enterobacteriaceae


Identification of positive ONPG Enterobacteriaceae

Identification of negative ONPG Enterobacteriaceae


Identification of negative ONPG Enterobacteriaceae