MIC Test Strip | Etest | Principle | Reading




Principle

It is a thin, inert, non-porous plastic strip with a g / mL MIC reading scale on one side, and a preset antibiotic gradient on the other side.

When applied to a seeded agar surface, the preformed antibiotic gradient immediately transfers to the agar to form a stable, continuous and exponential gradient of antibiotic concentrations below the strip.

Example:

  • Etest® :developed by AB Biodisk, Solna, Sweden, and currently commercialized by bioMérieux,
  • MIC Test Strip® : developed by LIOFILCHEM, Italy

Procedure



Reading CMI strips


After 18 hours of incubation or more, a symmetrical inhibition ellipse centered along the strip is formed. The MIC is read directly from the g / mL scale at the point where the edge of the inhibition ellipse intersects the MIC test strip.



References:

- Journal of Clinical Microbiology : First Comprehensive Evaluation of the MIC Evaluator Device

- MIC Test Strip Technical Sheet GRD - MTS28 - Rev.2.1 / 11.04.2016

- ETEST® REading guidE : AB BIODISK

- Thermo Scientific™ Oxoid™ M.I.C.Evaluator™ (M.I.C.E.™) Strips Step-by-Step Guide

- MIC Test Strip Italian Patent no. 1395483, International Patent Pending

- www.biomerieux-usa.com • @biomerieuxUSA

- MIC Test Strip for antimicrobial susceptibility testing