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HEPES is a general purpose buffering agent widely used in biochemistry and molecular biology to maintain physiological pH (pH range 6.8-8.2).
It is a zwitterionic organic compound, it tends to capture or release H+ ions depending on the pH value of the aqueous solution where they are found. The zwitterion is in equilibrium with the cation and the corresponding anion.
HEPES Buffer is categorized as "Good buffers", which is derived from a set of buffers described by Dr. Norman Good et al. (Good et al., Biochemistry 1966).
Hepes buffer
Hepes free acid | |||||||||
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CAS number | 7365-45-9 | ||||||||
Molecular mass | 238.30 g/Mol | ||||||||
Formula | C8H18N2O4S | ||||||||
Synonyms | N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic) acid |
HEPE sodium saltS | |||||||||
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CAS number | 75277-39-3 | ||||||||
Molecular mass | 260.29 g/Mol | ||||||||
Formula | C8H17N2O4SNa | ||||||||
Synonyms | sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate |
A buffer is a solution that contains an acid and a base or a salt. Its main purpose is to maintain the concentration of hydrogen ions at an ideal level for specific purposes, such as a bacterial culture. Just like other types of chemical buffers, HEPES buffer maintains the ion concentration of a solution at an ideal level (= ideal pH level) for a certain use. Specifically, HEPES buffer is used to maintain ideal conditions for cell culture
HEPES is a general purpose buffer. It is water soluble, does not interfere with biological processes or biological membranes (penetration, solubilization, surface adsorption, etc.), has a known tendency to form complexes with metal ions, is non-toxic and has very low U.V. absorption at wavelength > 260 nm.
HEPES powder is high temperature resistant with its melting point reaching 200℃, so it will not degrade by autoclaving, or you can filter the HEPES buffer solutions using a syringe filter from 0.2 micrometer.
The aqueous solution of HEPES should be stored away from light. exposure to ambient light for three hours would produce cytotoxic hydrogen peroxide
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