Acinetobacter Gram stain

Tips to differentiate acinétobacter

❶ Biochemical differentiation by TSI agar

Acinetobacter do not ferment glucose, and are strictly aerobic, so they will not change the color of the TSI medium, unlike, for example, Staphylococcus which performs acid fermentation..

❷ Exponential phase stimulation

To better appreciate the bacterial coloration, it is advisable to put colonies in a nutrient broth and incubate it in a water bath for 30min to 1 hour..

❸ Potassium hydroxide (KOH) string Test


- European Society of Clinical Microbiology and Infectious Diseases : Acinetobacter ursingii masquerading as Gram-positive cocci

- The Open Microbiology Journal ; Misinterpretation of Gram Stain from the Stationary Growth Phase

- ASM journals