☰ Summary :
In 1886, methylene blue was used for the first time in the medical field. Dr Paul Ehrlich discovered a strange phenomenon in an experiment: Methylene blue stained living neurons blue, and it has the same effect on Plasmodium (a parasite that causes malaria) in human blood.
Currently, it is widely used in chemical indicators, dyes, biological dyes and drugs
Methylene blue stain is a fast, economical and commonly used stain.
Methylene blue, or methylthioninium chloride, is an organic, odorless crystalline solid soluble in water and, to a lesser extent, in ethanol. In its pure state, it comes in the form of a dark green powder, it is also found commercially as a double salt with zinc chloride, brown in color.
Methylene blue is an oxidation-reduction agent (depends on its redox state: it is colorless in the reduced state, but is blue in the oxidized state).
◈ Methylene Blue solution according to Loeffler : Methylene blue: 0.30 g + Ethyl alcohol 95% : 30.0 ml + Distilled water 100.0 ml
Since cells usually have negatively charged cell walls, the chromophores of basic, positively charged dyes tend to stick to cell walls, making them positive spots.
Methylene blue stain is a simple stain where a single dye is used to emphasize particular structures in the sample, shape (the size and arrangement of bacteria). The organisms in a sample will be the same color, even if the sample contains more than one type of organism.
◈ In some cases, the methylene blue stain gives better results than the Gram stain. Among these cases :
y.pestis "security pin" aspect
Pour a methylene blue solution onto a smear
After 1-3min rinse with tap water
Dry between two sheets of blotting paper then observed by immersion
Place a small drop of faecal mucus or liquid stool on a microscopic slide. Add an equal volume of methylene blue (Loeffler blue). Mix with a small wooden stick; cover with a strip. Leave the coloring to take place for 2 to 3 minutes. Examine with the dry objective (× 40 or × 60).
The stainable structures appear blue, the staining makes it possible to detect the morphology of these structures, their grouping mode and in some cases the cell type
Yeasts stained with Methylene blue