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Buffers are aqueous systems that resist changes in pH when small amounts of acid or base are added. Buffer solutions are composed of a weak acid (the proton donor) and its conjugate base (the proton acceptor).
Bicine is a zwitterionic buffer used in biochemistry and molecular biology, it is widely used as a buffering agent for low temperature work over a pH range of 7.6 to 9.0.
Bicine bufer
The bicine buffer is categorized as "Good buffers", which derive from a set of buffers described by Dr. Norman Good et al. (Good et al., Biochemistry 1966).
BICINE buffer |
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CAS number | 150-25-4 | ||||||||
Molecular mass | 163.2 g/Mol | ||||||||
Formula | C6H13NO4 | ||||||||
Synonyms | N,N-bis(2-hydroxyethyl)glycine | ||||||||
pH range | 7.6 – 9.0 | ||||||||
pKa at 25°C | 8.26 |
Bicine Buffer is commonly used in protein crystallization solutions, it is also used in thin layer ion exchange chromatography for protein resolution, as well as in a multiphase buffer system for SDS-PAGE of proteins and has been reported to give better protein resolution and representation than using glycine or tricine
Bicine is commonly used as a buffer in the polymerase chain reaction and for the extraction of enzymes from various plant species.
It is soluble in water at 0°C. Bicine forms a complex with most common metals, stability constants and concentrations should therefore be taken into account when choosing this buffer.