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🏾 Definition
Buffers are aqueous systems that resist changes in pH when small amounts of acid or base are added. Buffer solutions are composed of a weak acid (the proton donor) and its conjugate base (the proton acceptor).
Bicine is a zwitterionic buffer used in biochemistry and molecular biology, it is widely used as a buffering agent for low temperature work over a pH range of 7.6 to 9.0.
Bicine bufer
The bicine buffer is categorized as "Good buffers", which derive from a set of buffers described by Dr. Norman Good et al. (Good et al., Biochemistry 1966).
BICINE buffer |
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| CAS number | 150-25-4 | ||||||||
| Molecular mass | 163.2 g/Mol | ||||||||
| Formula | C6H13NO4 | ||||||||
| Synonyms | N,N-bis(2-hydroxyethyl)glycine | ||||||||
| pH range | 7.6 – 9.0 | ||||||||
| pKa at 25°C | 8.26 | ||||||||
🏾 Bicine tampon applications and uses
Bicine Buffer is commonly used in protein crystallization solutions, it is also used in thin layer ion exchange chromatography for protein resolution, as well as in a multiphase buffer system for SDS-PAGE of proteins and has been reported to give better protein resolution and representation than using glycine or tricine
Bicine is commonly used as a buffer in the polymerase chain reaction and for the extraction of enzymes from various plant species.
🏾 Properties
It is soluble in water at 0°C. Bicine forms a complex with most common metals, stability constants and concentrations should therefore be taken into account when choosing this buffer.